Advanced glycation end-products in corneas of patients with keratoconus
- 7 March 2002
- journal article
- Published by Springer Nature in Albrecht von Graefes Archiv für Ophthalmologie
- Vol. 240 (4) , 296-301
- https://doi.org/10.1007/s00417-002-0445-3
Abstract
Background: Keratoconus remains a poorly understood yet widespread disease which poses a potential threat to human vision. The underlying mechanisms are still not clear. One possible pathway is increased formation and accumulation of advanced glycation end-products (AGEs) in the cornea. Methods: Corneas obtained from six patients with keratoconus and from six healthy controls were investigated. An immunohistochemical localisation of the well-known AGE Nε-carboxymethyllysine (CML) was performed using a polyclonal anti-CML antibody. Results: In the corneas of all six patients with keratoconus, CML immunoreactivity was found in the epithelial as well as in the endothelial cells. The keratocytes also showed a positive reaction. The controls, in contrast, showed very little or no immunoreactivity. Conclusion: In the epithelial, stromal and endothelial cells of corneas with keratoconus an accumulation of CML was detected which might play a role in the pathogenesis of keratoconus.Keywords
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