The β-glucosaminase activity of testicular extracts and its bearing on the problem of diffusing factors

Abstract
Diffusing factor concentrates from bull testicle and from Crotalus atrox venom contained a specific [beta]-glucosaminase. This enzyme hydrolyzed [beta]-phenyl-N-acetyl glucos-amide. The pH optimum was 4.6. The glucosaminase was not identical with the agent which rapidly decreased the viscosity of glucosamine containing mucopolysaccharides of the hya-luronic acid type.