Immunoassay of 6-Hydroxymelatonin Sulfate in Human Plasma and Urine: Abolition of the Urinary 24-Hour Rhythm with Atenolol*

Abstract
An assessment of the rhythmic characteristics of melatonin secretion in manand other species requires the determination of 24-h secretion profiles. Measurement of a major excreted metabolite would allow noninvasive study of pineal function, applicablein particular to pediatric and long term circadian rhythm studies. This report describesa simple and rapid RIA for 6-hydroxymelatonin sulfate in human plasma and urine. Physiological studies revealed that both plasma and urinary levels of 6-hydroxymelatonin sulfate were closely related to plasma melatonin, and that the urinary 24-h rhythm was abolished by the β1-adrenergic anagonist atenolol. {J Clin Endocrinol Metab60:1166, 1985)

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