Confirmation of the human cathepsin B gene (CTSB) assignment to chromosome 8
- 1 April 1992
- journal article
- Published by Springer Nature in Human Genetics
- Vol. 89 (1) , 10-12
- https://doi.org/10.1007/bf00207033
Abstract
Human cathepsin B gene (CTSB) has been mapped to two locations: 8p22 and 13q14. Here we confirm the chromosome 8 assignment by three independent methods: (1) analysis of human-hamster somatic cell hybrid DNA by polymerase chain reaction; (2) comparison of hybridization signals to cathepsin B in interphase nuclei of normal fibroblasts and fibroblasts with a chromosome 8 deletion; and (3) fluorescence in situ hybridization to metaphase spreads using cathepsin B cosmid clones. Our results indicate that human CTSB is located at 8p22-p23.1.Keywords
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