Measurement of cellular elastic properties by acoustic microscopy
- 1 June 1984
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 134 (3) , 245-260
- https://doi.org/10.1111/j.1365-2818.1984.tb02518.x
Abstract
The acoustic microscope is used to investigate the elastic properties of living biological cells. A quantitative model is developed relating acoustic microscope image contrast to cellular elastic properties. Cytoplasmic acoustic attentuation is measured by focusing the acoustic microscope on the surface of the underlying substrate. Cytoplasmic acoustic impedance is measured by focusing the acoustic microscope on the top surface of the cell. The model allows the acoustic microscope to give quantitative information about cellular elasticity on a subcellular scale.Keywords
This publication has 11 references indexed in Scilit:
- Acoustic microscopy of living cells.Proceedings of the National Academy of Sciences, 1981
- Acoustic Microscopy of Slowness SurfacesPublished by Institute of Electrical and Electronics Engineers (IEEE) ,1981
- Dependence of the ultrasonic properties of biological tissue on constituent proteinsThe Journal of the Acoustical Society of America, 1980
- Acoustic microscopy: resolution of subcellular detail.Proceedings of the National Academy of Sciences, 1979
- Reflection coefficient for a lossy liquid–lossless isotropic solid interfaceThe Journal of the Acoustical Society of America, 1979
- Acoustic microscopy with mechanical scanning—A reviewProceedings of the IEEE, 1979
- An angular-spectrum approach to contrast in reflection acoustic microscopyJournal of Applied Physics, 1978
- Phase imaging in reflection with the acoustic microscopeApplied Physics Letters, 1977
- Transmission and reflection of ultrasonic waves in layered mediaThe Journal of the Acoustical Society of America, 1977
- The locomotion of fibroblasts in cultureExperimental Cell Research, 1971