Tissue Culture, a New Tool for Propagating and Breeding Rape and Other Plants

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Abstract
During sexual propagation of rape varieties new genetic combinations arise in the offspring, and thus valuable characteristics can be modified or lost in successive generations. If and when it will become possible to propagate such new varieties vegetatively through their tissue cultures, desired properties can be maintained in successive generations, because vegetative propagation does not involve gametic fusion. Experimental conditions employed to induce “organogenesis” and “embryogenesis” in cells of plant tissue cultures, especially of rape cultures are specified. New varieties may be produced by inducing mutation in cells of rape tissue cultures, by fusion of protoplasts from rape or from rape and other plants, by establishing haploid cultures from pollen grains of rape and inducing organogenesis in the cells, and by programming rape cells through introducing genetic information in the protoplasts.