Culture ofChara hispida

Abstract
Culture conditions that support high growth rates of Chara hispida L and Nitella flexilis (L.) Ag. are described. The optimum conditions for both rhizoid and shoot growth are obtained using a substrate of 425–710 μm sand particles and a solution of inorganic salts in distilled water. The highest rates of linear extension and dry weight increase of shoots are obtained with sand substrates less than 710 μm in size.