Identification of nonhistone chromatin proteins in chromatin subunits (or mononucleosomes) devoid of histone H1

Publisher Website
Google Scholar
Abstract
Rat liver chromatin was digested by micrococcal nuclease. Chromatin subunits (or mononucleosomes) were isolated by sucrose density gradient and subsequently fractionated by 6% polyacrylamide gel electrophoresis into 2 major components. One component (MN1) of the mononucleosomes had a higher mobility, contained histones H2A, H2B , H3, H4 and shorter DNA fragments (140 base pairs) while the other (MN2) contained all 5 histones and longer DNA fragments (180 base pairs). Both submononucleosomes (MN1 and MN2) contained nonhistone chromatin proteins (NHCP). By electrophoresis in 15% sodium dodecyl sulfate-polyacrylamide gel, 9 and 11 major fractions of NHCP were identified in the submononucleosomes MN1 and MN2, respectively. Treatment of mononucleosomes with 0.6 M NaCl removes most of the NHCP (except for 2 major NHCP which remain in the core particles) and histone H1.

This publication has 3 references indexed in Scilit: