Multifunctional properties of beef liver phosphoglucomutase.
- 1 January 1976
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 40 (12) , 2433-2439
- https://doi.org/10.1271/bbb1961.40.2433
Abstract
Liver phosphoglucomutase was found to catalyze also the reaction of Glc-1, 6-P2 formation from Glc-1-P and Fru-1, 6-P2 or Glc-1-P and glycerate-1, 3-P2. The specific activity of Glc-1, 6-P2 formation from Glc-1-P and Fru-1, 6-P2 was 1/9200 of that of the mutase activity. The activity of Glc-1, 6-P2 formation from Glc-1-P and glycerate-1, 3-P2 was 1/122, 000 of the mutase activity. From the results of the kinetics and the thermal inactivation experiments, the reaction of the mutase and Glc-1, 6-P2 synthesis were strongly suggested to occur at the same active site of liver phosphoglucomutase. Liver phosphoglucomutase exhibited the Glc-1, 6-P2 phosphatase activity only in the presence of xylose 1-phosphate. The specific activity of phosphatase was only 1/154, 000 of that of the mutase activity.Keywords
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