VISUALIZATION AND MORPHOLOGY OF PLEUROPNEUMONIALIKE ORGANISMS IN CLINICAL MATERIAL

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Abstract
Intracytoplasmic inclusions seen within epithelial cells from cases of nongonococcal urethritis in human males are interpreted to be the tissue-phase of T strain pleuropneumonialike organisms (PPLO). They exhibit structures that are consistent in size, morphology, and staining reaction to those of the pleuropneumonia group as a whole. They are characterized by minute size and characteristic polymorphic structures and by their staining reaction in Gierosa or azure -eosinate stained epithelial scrapings. Characteristic particle aggregations and viruslike inclusions were formed. Demonstration of intracytoplasmic PPLO inclusions in stained epithelial scrapings from individuals yielding only T strain PPLO in culture, provides additional evidence that T strain PPLO are newly recognized members of the pleuropneumonia group organisms. Following successful treatment, PPLO inclusions could no longer be demonstrated in stained epithelial scrapings, nor could T strain pleuropneumonialike organisms again be recovered in culture.