EFFECT OF T-LYMPHOCYTE AND B-LYMPHOCYTE DEPLETION ON PROTECTION OF MICE VACCINATED WITH A GAL-E MUTANT OF SALMONELLA-TYPHIMURIUM

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 57  (3) , 354-360
Abstract
Immunosuppressive agents were used to determine the relative importance of T [thymus-derived] and B [bone marrow-derived] lymphocytes in conferring protection to mice vaccinated with a live gal E mutant of S. typhimurium, strain G30D. Lymphocyte transformation and serum agglutination tests showed that while cyclophosphamide (CPA) suppressed B lymphocytes, [horse] antilymphocyte sera (ALS) suppressed T and B cells. The humoral response of vaccinated animals treated with ALS was supplemented by the i.v. injection of serum from untreated vaccinated mice. CPA-treated mice could not control multiplication of the vaccinal strain which eventually killed them. There was little multiplication of the vaccinal strain in the controls and ALS-treated mice, all of which survived to challenge. The vaccinated controls and vaccinated ALS treated groups each survived infection with the challenge strain which was gradually eliminated. Humoral immunity was of greater importance than cellular immunity in mice vaccinated i.p. with strain G30D.