POLYACRYLAMIDE GEL ELECTROPHORESIS OF RHESUS MONKEY (MACACA MULATTA) PITUITARY GLAND EXTRACTS

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Abstract
Primate growth hormone (GH) preparations possess high lactogenic activity apparently as an intrinsic property of the GH molecule (see Forsyth, 1968). However, cytological studies of human and monkey pituitary glands and organ culture experiments with foetal human pituitaries (Pasteels, 1963; Girod, 1964) suggest that a distinct primate prolactin may also be produced at least during pregnancy and lactation. To investigate this problem further, 15 pituitary glands from adult female rhesus monkeys, kindly supplied by the Department of Anatomy, University of Birmingham, have been investigated using polyacrylamide gel electrophoresis (PAGE). Six glands were from pregnant and nine from non-pregnant females. Of the latter, four were pre-ovulatory, three in the luteal phase and two had recently spontaneously aborted. All glands were stored at − 20° until required. Individual glands were extracted for 6 hr. at 3° in l ml. tris—HCl buffer (0·75 m-tris adjusted to pH 8·4 with 0·1 n-HCl).

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