Control ofAzospirillum brasilenseNifA activity by PII: effect of replacing Tyr residues of the NifA N-terminal domain on NifA activity
Open Access
- 1 October 1999
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 179 (2) , 339-343
- https://doi.org/10.1111/j.1574-6968.1999.tb08747.x
Abstract
It was previously reported that the N-terminal domain of Azospirillum brasilense NifA was a negative regulator of the NifA activity and that the PII protein prevented this inhibition under nitrogen fixing conditions. Here, we show that a mutation of a single Tyr residue at position 18 of the N-terminal domain of NifA led to an active NifA protein that did not require PII for activation under nitrogen fixation conditions.Keywords
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