Method for studying the development, structure and microflora of denture plaque

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Abstract
Pieces of sandblasted, self-adhesive tape were stuck to the fitting surface of maxillary dentures in 17 edentulous subjects with clinically normal palatal mucosa. After 1 wk, during which the subjects abstained from denture hygiene, the tape samples were removed for cultural and microscopical examinations. The microorganisms were suspended in reduced transport fluid by ultrasonic treatment and total viable anaerobic counts and viable counts of yeasts/cm2 of tape were calculated after inoculation of serial dilutions on nonselective medium and Sabouraud agar. Total anaerobic viable counts ranged from 4 .times. 104-5 .times. 108. By repeated sampling, the largest intra-individual variation was 10-fold. Yeast counts constituted < 1% of the total viable counts in all but 2 of the subjects. Light microscopy and EM showed a bacterial plaque of predominantly cocci and rods.

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