The nudeotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E. coil K12
- 25 June 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (12) , 2889-2903
- https://doi.org/10.1093/nar/9.12.2889
Abstract
We have determined the nudeotide sequence of the rpoD gene which codes for the sigma subunit of RNA polymerase from E. coli K12. The gene, which we formerly cloned as a Bindlll restriction fragment in the transducing phage, Charon 25, was recloned into several plasmids. We have determined a 2600 base pair DNA sequence which includes the entire structural gene for sigma. The resulting amino acid sequence agrees with previous information obtained about sigma including theamino acid composition, partial sequence data for the N-terminus, the highly acidic nature of the polypeptide, and the cleavage pattern at cysteines. The molecular weight of 70,263 daltons calculated for the 613 amino acid polypeptide is significantly lower than had been determined previously by SDS polyacrylamide gel analysis.Keywords
This publication has 39 references indexed in Scilit:
- Subunit topography of RNA polymerase from Escherichia coli. A cross-linking study with bifunctional reagentsBiochemistry, 1977
- A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.Proceedings of the National Academy of Sciences, 1977
- Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.Proceedings of the National Academy of Sciences, 1977
- Primary structure of α‐subunit of DNA‐dependent RNA polymerase from Escherichia coliFEBS Letters, 1977
- [13] Cleavage at cysteine after cyanylationPublished by Elsevier ,1977
- The Modification of DNA‐Dependent RNA Polymerase from Escherichia coli by an Alkylating Derivative of Rifamycin SVEuropean Journal of Biochemistry, 1975
- The subunits of DNA‐dependent RNA polymerase from E. coli: I. Amino acid analysis and primary structure of the N‐terminal regionsFEBS Letters, 1975
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975
- The Selectivity of TranscriptionAnnual Review of Biochemistry, 1974
- The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding SitesProceedings of the National Academy of Sciences, 1974