Escherichia coliTransformant Expressing the Glucose Dehydrogenase Gene fromBacillus megateriumas a Cofactor Regenerator in a Chiral Alcohol Production System
- 1 January 1998
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 62 (1) , 167-169
- https://doi.org/10.1271/bbb.62.167
Abstract
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP+ and glucose, similarly to commercially available GDH.Keywords
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