High-Performance Liquid Chromatographic Determination of Ethylenethiourea in Food

Abstract
An HPLC procedure was developed for the determination of ethylenethiourea (ETU) in peels of potatoes, tomatoes and apples after application of mancozeb suspension (ethyl-enebisdithiocarbamate fungicide). The analytical procedure consisted of a single extraction step with methanol and ETU was eluated from SEPARON CN column. Mobile phase was 4 % methanol in chloroform cyclohexane (1:1). The level of ethylenthiourea after application of mancozeb decreased slowly, with a half time six days in each case.