Isolation and analysis of ketoconazole resistant mutants ofSaccharomyces cerevisiae
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Medical Mycology
- Vol. 26 (3) , 153-162
- https://doi.org/10.1080/02681218880000231
Abstract
Nine mutants of Saccharomyces cerevisiae which are resistant to ketoconazole, have been isolated and characterized. In each case the mutation is nuclear in origin and allelic to previously described mutation, erg3, which gives rise to a block in the .DELTA.5-6 desaturation step of ergosterol biosynthesis. The significance of this second site mutation to the point of inhibitory action of ketoconazole, that is the P-450-mediated C-14 demethylation of lanosterol, is discussed.This publication has 23 references indexed in Scilit:
- The lipid composition and permeability to azole of an azole- and polyene-resistant mutant ofCandida albicansMedical Mycology, 1987
- Azole resistance inCandida albicansMedical Mycology, 1986
- Mitochondrial resistance to miconazole in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1985
- Differences in the cytochrome P-450 enzymes of sterol C-14 demethylase mutants of Saccharomyces cerevisiaeCurrent Genetics, 1985
- Chemotherapeutic and agrochemical applications of cytochrome P-450 ligandsTrends in Biochemical Sciences, 1984
- Azole resistance inCandida albicansMedical Mycology, 1984
- Sterol Structure and Membrane FunctionPublished by Elsevier ,1981
- Metabolism of Δ24-sterols by yeast mutants blocked in removal of the C-14 methyl groupCanadian Journal of Biochemistry, 1978
- Yeast mutants requiring ergosterol as only lipid supplementBiochemical and Biophysical Research Communications, 1974
- Polyene Resistance and the Isolation of Sterol Mutants in Saccharomyces cerevisiaeJournal of General Microbiology, 1972