Studies on the development and maintenance of epithelial cell surface polarity with monoclonal antibodies.
Open Access
- 1 May 1984
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 98 (5) , 1777-1787
- https://doi.org/10.1083/jcb.98.5.1777
Abstract
Epithelial cell surface polarity in subconfluent and confluent Madin-Darby canine kidney (MDCK) cells with monoclonal antibodies directed against plasma membrane glycoproteins of 35,000, 50,000 and 60,000 MW. The cell surface distribution of these glycoproteins was studied by immunofluorescence and immunoelectron microscopy. At the ultrastructural level, the electron-dense reaction product localizing all 3 glycoproteins was uniformly distributed over the apical and basal cell surfaces of subconfluent MDCK cells as well as on the lateral surfaces between contacted cells; however, after formation of a confluent monolayer, these glycoproteins could only be localized on the basal-lateral plasma membrane. The development of cell surface polarity was followed by assessing glycoprotein distribution with immunofluorescence microscopy at selected time intervals during growth of MDCK cells to form a confluent monolayer. These results were correlated with transepithelial electrical resistance measurements of tight junction permeability, and it was determined by immunofluorescence that polarized distributions of cell surface glycoproteins were established just after electrical resistance could be detected, but before the development of maximal resistance. Intact tight junction may be required for the establishment of the apical and basal-lateral plasma membrane domains, and development of epithelial cell surface polarity is a continuous process.This publication has 54 references indexed in Scilit:
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