Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells
- 10 November 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 208 (1) , 48-51
- https://doi.org/10.1016/0014-5793(86)81529-0
Abstract
A rise of cytosolic Ca2+ is induced by NGF in rat pheochromocytoma PC12 and bovine chromaffin cells investigated (both in suspension and while attached to polyornithine‐coated glass slides) by fluorescence techniques (with quin‐2 and fura‐2). The effect of NGF on [Ca2+]i is delayed (30–40 s of lag phase), slow (tf1/2 = 40 s), relatively small (+50‐75%) and persistent (over 10 min). It is due to Ca2+ influx (requires extra‐cellular Ca2+ > 10 μM) through a pathway different from the voltage‐gated Ca2+ channel, possibly accompanied by intracellular Ca2+ redistribution, and might play a messenger role in NGF action.Keywords
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