High-Performance Liquid Chromatographic Analysis of Citric ACID, Inositol and Fructose in Boar and Bull Seminal Plasma

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Abstract
The efficiency of ultrafiltration and high-performance liquid chromatography (HPLC) in the routine analysis of dialysable sugar alcohols, organic acids and carbohydrates in boar and bull seminal plasma is evaluated. Permitting a simple and rapid separation of protein-free samples, ultrafiltration reduces the time of HPLC analysis by 40 min as compared to protein precipitation by means of methanol. Under optimal flow-conditions the separation of citric acid, inositol and fructose is achieved in 16 min. The use of silicon-oil for separating seminal plasma within seconds is described.