Acidified Glycerol Lysis Test: a Screening Test for Spherocytosis

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Abstract
Summary Acidified glycerol lysis test (AGLT) is a screening procedure which has been developed for spherocytosis. AGLT was found positive in 100% of 48 patients suffering from hereditary spherocytosis, 100% of nine couples of affected parents, and 86% of 14 couples of clinically healthy parents. The test was positive in acquired spherocytosis and negative in normal controls. AGLT appears to have a predictive value higher than the entire battery of conventional tests. It is simple, rapid, inexpensive, gives clear‐cut results and requires minute amounts of blood. It can be performed on blood stored for up to 24 h.

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