Acute porphyria. I. some observations on the solubility, precipitation, fluorescense and absorbtion spectra of calcium phosphate-adsorbed porphyrin pigments

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Abstract
I. Urine from an acute porphyric was boiled 30 min. at pH 5.0-5.5 and the pigments present were adsorbed to Ca phosphate. The precipitate formed was not completely soluble in concentrated HC1. The HC1-soluble pigments were precipitated in different ways, using NaOH NH4OH, and Na acetate. Dilute NH4OH gave the best separation of the porphyrins and a pigment with a porphobilin-like absorption spectrum. II. The conversion products from porphobilinogen were separated, esterified and crystallized from methanol. The color, fluorescence and absorption spectrum of the methyl esters in CHCl3 soln. were examined. The metal-binding in the metallo-porphyrins was found to be extraordinarily firm. III. An ether/ glacial acetic acid-extractable coproporphyrin chromogen was shown to be present in normal and pathological urine. In some cases up to 2/3 of the total coproporphyrin is excreted as the chromogen. The nature of the chromogen is discussed.
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