Endogenous Moloney leukemia virus in nonviremic Mov substrains of mice carries defects in the proviral genome

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Abstract
Substrains of mice carrying Moloney murine luekemia virus as a Mendelian gene (Mov locus) have been derived previously. Some of these strains, i.e., Mov-3 and Mov-9, develop viremia; others, i.e., Mov-2, Mov-7 and Mov-10, do not regularly activate virus. The respective Mov loci were previously molecularly cloned and the proviral clones derived from the different viral loci were either infectious (Mov-3, Mov-9) or failed to induced infectious virus (Mov-2, Mov-7, Mov-10) in a transfection assay. To analyze the sites affecting infectivity of the latter clones, complementation assays, in vitro recombinations and marker rescue experiments were performed. Evidently, the 3 endogenous Moloney murine leukemia virus clones derived from Mov-2, Mov-7 and Mov-10 carry different mutations in the gag-pol region of the proviral genome. No inhibitory effect of flanking mouse sequences on provirus infectivity was observed.