Determination of Protein−Ligand Association Thermochemistry Using Variable-Temperature Nanoelectrospray Mass Spectrometry
- 25 March 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (15) , 4786-4787
- https://doi.org/10.1021/ja0316972
Abstract
A novel temperature-controlled nanoelectrospray (nanoES) device, interfaced with a Fourier transform ion cyclotron resonance mass spectrometer, is used to measure the association constants, Kassoc, for a series of protein−carbohydrate complexes at solution temperatures ranging from 5 to 40 °C. From a van't Hoff analysis of the Kassocvalues, the enthalpies and entropies of association (ΔHassoc, ΔSassoc) at 25 °C are determined. The nanoES/mass spectrometry-derived thermodynamic parameters are in agreement with values previously determined by isothermal titration calorimetry.Keywords
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