Delivery and Expression of Functional Viral RNA Genomes In Planta by Agroinfiltration
Open Access
- 1 June 2006
- journal article
- unit
- Published by Wiley in Current Protocols in Microbiology
- Vol. 1 (1) , 16B.2.1-16B.2.15
- https://doi.org/10.1002/9780471729259.mc16b02s01
Abstract
Agroinfiltration is a simple, efficient, and powerful approach for transient expression of viral genes as well as DNA-based expression of full-length RNA genomes of plant viruses for studies leading to understanding of replication, movement, and assembly. Most importantly, it results in synchronous delivery of Agrobacterium transformants to a majority of cells encompassing the infiltrated area and is therefore ideal for examining the biological activities of viruses having multipartite genomes. Because of the high transformation rate and efficient accumulation of mRNAs, the method is also ideal for analyzing biological activities of viral genomes with defective replication and cell-to-cell movement characteristics.Keywords
This publication has 21 references indexed in Scilit:
- Replication-independent expression of genome components and capsid protein of brome mosaic virus in planta: A functional role for viral replicase in RNA packagingVirology, 2005
- Arabidopsis thaliana has the enzymatic machinery for replicating representative viroid species of the family PospiviroidaeProceedings of the National Academy of Sciences, 2004
- Role of the 3′-Untranslated Regions of Alfalfa Mosaic Virus RNAs in the Formation of a Transiently Expressed Replicase in Plants and in the Assembly of VirionsJournal of Virology, 2001
- Silencing on the Spot. Induction and Suppression of RNA Silencing in the Agrobacterium-Mediated Transient Expression SystemPlant Physiology, 2001
- Agroinfiltration of Cauliflower mosaic virus Gene VI Elicits Hypersensitive Response in Nicotiana SpeciesMolecular Plant-Microbe Interactions®, 2000
- Fast forward genetics based on virus-induced gene silencingCurrent Opinion in Plant Biology, 1999
- An Agrobacterium-mediated transient gene expression system for intact leavesPlant Science, 1997
- Cis- and trans-acting ribozymes from a human pathogen, hepatitis delta virusTrends in Biochemical Sciences, 1994
- Infectious Transcripts and cDNA Clones of RNA VirusesVirology, 1994
- Agrobacterium-mediated delivery of infectious maize streak virus into maize plantsNature, 1987