Genotypic Differences in Morphogenic Capacity of Cultured Leaf Explants of Tomato

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Abstract
Cultured leaf sections of 12 cultivars of tomato, Lycopersicon esculentum Mill., were evaluated concerning their morphogenic responses to 24 combinations of indoleacetic acid (IAA, 0–2.0 mg/liter) and 6-benzylamino purine (BA, 0–10 mg/liter). Morphogenic responses were cultivar-dependent and exhibited broad maxima over a range of growth regulator concentrations. Media containing 0.2 mg/liter IAA and 1.0 or 5.0 mg/liter BA were optimal callus induction media for most cultivars. Leaf explants of all cultivars rooted on a medium supplemented only with 0.2–2.0 mg/liter IAA. Genotypic differences were observed in ability to regenerate shoots and average number of shoots regenerated. Optimal shoot regeneration medium varied with cultivar, but most often contained 0.2 or 1.0 mg/liter IAA + 2.5 or 5.0 mg/liter BA. ‘Better Boy’, ‘Starfire’, and ‘UC 134-1-2’ regenerated shoots most readily, averaging more than 10 shoots per culture. ‘Starfire’ was most amenable to in vitro shoot regeneration with as many as 19 shoots per culture.

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