Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase
- 1 June 1988
- Vol. 23 (6) , 605-608
- https://doi.org/10.1007/bf02535605
Abstract
A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic‐2,3(1,2)‐dioleoyl‐sn‐glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic triacylglycerol lipase activities in post‐heparin plasma. Optimal conditions for the determination of the two post‐heparin plasma lipases were similar to those using radiolabeled triolein. Using this substrate, both post‐heparin lipases exhibited their characteristic properties (pH optimum and effect of inhibitors) and attacked external ester bonds (1 or 3) containing pyrene decanoic and oleic acids at a similar rateKeywords
This publication has 23 references indexed in Scilit:
- 1,2-Dipalmitoyl-3-β-2-furylacryloyltriacylglycerol: a chromophoric substrate for lipoprotein lipaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- New fluorometric assay of lysosomal acid lipase and its application to the diagnosis of wolman and cholesteryl ester storage diseasesClinica Chimica Acta; International Journal of Clinical Chemistry, 1985
- Effect of heparin-induced lipolysis on the distribution of apolipoprotein e among lipoprotein subclasses. Studies with patients deficient in hepatic triglyceride lipase and lipoprotein lipase.Journal of Clinical Investigation, 1985
- Lipolytic Enzymes and Plasma Lipoprotein MetabolismAnnual Review of Biochemistry, 1980
- Stereospecificity of lipoprotein lipase is an intrinsic property of the active site of the enzyme proteinFEBS Letters, 1978
- Simple Method for the Esterification of Carboxylic AcidsAngewandte Chemie International Edition in English, 1978
- Stereospecificity of hepatic lipasesFEBS Letters, 1976
- Effect of serum and C‐apoproteins from very low density lipoproteins on human postheparin plasma hepatic lipaseFEBS Letters, 1976
- Stereospecificity of lipases. enzymatic hydrolysis of enantiomeric alkyldiacyl- and dialkylacylglycerols by lipoprotein lipaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
- Stereospecificity of lipases. Enzymic hydrolysis of enantiomeric alkyl diacylglycerols by lipoprotein lipase, lingual lipase and pancreatic lipaseFEBS Letters, 1974