6‐AMINODOPAMINE‐INDUCED DEGENERATION OF CATECHOLAMINE NEURONS

Publisher Website
Google Scholar
Abstract
The effects of 6‐aminodopamine on central and peripheral catecholamine neurons using fluorescence histochemical and isotope techniques have been investigated. Systematic administration of 6‐aminodopamine (20 mg/kg intraveneously) produced a rapid (within 1 h) and long‐lasting depletion of endogenous noradrenaline in adrenergic nerves of mouse atrium and iris with a concomitant loss of [3H]noradrenaline uptake. The effects were dosedependent. Accumulations of noradrenaline in non‐terminal axons were observed histochemically, indicating that 6‐aminodopamine induces neuronal damage. Desipramine completely blocked the 6‐aminodopamine induced noradrenaline depletion and reduction in [3H]noradrenaline uptake, indicating that 6‐aminodopamine has to be taken up by the axonal ‘membrane pump’ to produce its effects. Themonoamine oxidase inhibitor, nialamide, potentiated the effect of 6‐aminodopamine on [3H]noradrenaline uptake. 6‐Aminodopamine did not affect the cell bodies of the adrenergic neurons and there was a reappearance of adrenergic nerves and recovery of [3H]noradrenaline uptake. 6‐Aminodopamine does not seem to pass the blood‐brain barrier after systemic injection.Intraventricular injection of 6‐aminodopamine in rats led to a considerable reduction in endogenous whole brain noradrenaline and [3H]noradrenaline uptake in slices from cerebral cortex and hypothalamus. Similar, but less pronounced effects were observed on dopamine neurons in the caudate nucleus. Histochemically, pronounced accumulations of transmitter were observed in the axons of the catecholamine neurons. The results obtained favour the view that 6‐aminodopamine is able to produce an acute and selective degeneration of catecholamine neurons similar to that seen after the neurotoxicagent, 6‐hydroxydopamine. Both compounds seemed to be approximately equally potent in their neurotoxicity, although 6‐aminodopamine seemed to be more generally toxic.