Nuclear factors specifically bind to upstream sequences of aXenopus laevisribosomal protein gene promoter
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (20) , 8171-8184
- https://doi.org/10.1093/nar/17.20.8171
Abstract
The upstream of the Xenopus laevis L14 ribosomal protein gene was deleted starting from the 5'' extremity in order to define the promoter length necessary to express a linked reporter CAT gene. The functional analysis indicated that a sequence located between -63 and -49 from the capsite is important for an efficient promoter activity. Band shift and ExoIII protection assays evidenced the binding to this region of a factor, called XrpFI, present in the crude nuclear extract from X. laevis oocytes. Methylation interference analysis localized the contacts in the G residues belonging to a short box, 5'' CTTCC 3'', positioned between -53 and -49 from the capsite. An additional factor, XrpFII, makes contacts with the sequence 5''GCCTGTTCGCC 3'' located between -27 and -17 from the capsite. The deletion mutant still containing this sequence is poorly transcribed, but resumes activity when a short fragment containing the binding site for factor XrpFI is cloned in an upstream position.This publication has 25 references indexed in Scilit:
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