MORPHOMETRIC ANALYSIS OF YEAST CELLS: III. SIZE DISTRIBUTION OF 2-DEOXYGLUCOSE-INDUCED LYSING SCHIZOSACCAROMYCES POMBE CELLS AND THEIR SITES OF LYSIS

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Abstract
To cultures of Schizosaccharomyces pombe, 2-deoxyglucose (2DG) was added, either as 7 μg/ml during inoculation of the cultures (low dosage), or as 250 μg/ml during the log phase (high dosage). Samples were removed from the cultures, and lysing and non-lysing cells were measured and tabulated. Addition of the high dosage was followed immediately by lysis, with over 85% of the lysing cells found in cytolysis at their primary growing ends Lysis ensued only at the beginning of the stationary phase in the low dosage experiments; 64% of the affected cells lysed at their cell plates. Cells lysing at their primary ends (high dose experiments) were shorter than the controls; cells lysing at their cell plates (low dose experiments) were longer than the controls. The cell division process of the last cell cycle completed in the culture is unusual in its susceptibility to the low initial dose of 2DG, suggesting that cell division metabolism is fundamentally different from wall extension metabolism in the fission yeast.