DISTINGUISHING BETWEEN OVINE ABORTION AND OVINE ARTHRITIS CHLAMYDIA-PSITTACI ISOLATES WITH SPECIFIC MONOCLONAL-ANTIBODIES

  • 1 July 1986
    • journal article
    • research article
    • Vol. 47  (7) , 1520-1523
Abstract
Monoclonal antibodies to Chlamydia psittaci were prepared by both in vivo and in vitro immunization methods, using an abortion strain of C. psittaci as the immunizing antigen. Seven of the 8 monoclonal antibodies produced were genus-specific by the enzyme-linked immunosorbent assay and immunofluorescence test. The genus-specific antibodies were reactive with a protease-resistant, periodate-sensitive antigen of < 14 kilodaltons. The remaining monoclonal antibody, 10D7, was specific for ovine abortion strains of C. psittaci and nonreactive with 2 strains isolated from the joints of lambs with polyarthritis. This type-specific antigen was protease sensitive, but could not be detected in the immunoblot assay.