Stoichiometry of Antibody Neutralization of Human Immunodeficiency Virus Type 1
Open Access
- 15 March 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (6) , 3350-3357
- https://doi.org/10.1128/jvi.79.6.3500-3508.2005
Abstract
The human immunodeficiency virus envelope glycoproteins function as trimers on the viral surface, where they are targeted by neutralizing antibodies. Different monoclonal antibodies neutralize human immunodeficiency virus type 1 (HIV-1) infectivity by binding to structurally and functionally distinct moieties on the envelope glycoprotein trimer. By measuring antibody neutralization of viruses with mixtures of neutralization-sensitive and neutralization-resistant envelope glycoproteins, we demonstrate that the HIV-1 envelope glycoprotein trimer is inactivated by the binding of a single antibody molecule. Virus neutralization requires essentially all of the functional trimers to be occupied by at least one antibody. This model applies to antibodies differing in neutralizing potency and to virus isolates with various neutralization sensitivities. Understanding these requirements for HIV-1 neutralization by antibodies will assist in establishing goals for an effective AIDS vaccine.Keywords
This publication has 133 references indexed in Scilit:
- Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virionsProceedings of the National Academy of Sciences, 2003
- Envelope Glycoprotein Incorporation, Not Shedding of Surface Envelope Glycoprotein (gp120/SU), Is the Primary Determinant of SU Content of Purified Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency VirusJournal of Virology, 2002
- Additive Effects Characterize the Interaction of Antibodies Involved in Neutralization of the Primary Dualtropic Human Immunodeficiency Virus Type 1 Isolate 89.6Journal of Virology, 2001
- CD4-dependent, antibody-sensitive interactions between HIV-1 and its co-receptor CCR-5Nature, 1996
- Membrane fusion mediated by the influenza virus hemagglutinin requires the concerted action of at least three hemagglutinin trimers.The Journal of cell biology, 1996
- The fusion kinetics of influenza hemagglutinin expressing cells to planar bilayer membranes is affected by HA density and host cell surface.The Journal of general physiology, 1995
- Identification of Conserved and Variant Epitopes of Human Immunodeficiency Virus Type 1 (HIV-1) gp120 by Human Monoclonal Antibodies Produced by EBV-Transformed Cell LinesAIDS Research and Human Retroviruses, 1990
- Formation of mixed hemagglutinin trimers in the course of double infection with influenza viruses belonging to different subtypesVirus Research, 1988
- Posttranslational oligomerization and cooperative acid activation of mixed influenza hemagglutinin trimers.The Journal of cell biology, 1988
- Shedding and Interspecies Type Sero-reactivity of the Envelope Glycopolypeptide gp120 of the Human Immunodeficiency VirusJournal of General Virology, 1986