AGE-RELATED DECLINE IN LYSOSOMAL-ENZYME RELEASE FROM POLYMORPHONUCLEAR LEUKOCYTES AFTER N-FORMYL-METHIONYL-LEUCYL-PHENYLALANINE STIMULATION

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 11  (10) , 1005-1013
Abstract
Aging is assumed to decrease lysosomal enzyme release from polymorphonuclear leukocytes (PMN). A synthetic chemotactic peptide, fMet-Leu-Phe was utilized to stimulate enzyme release of PMN from 45 human subjects, 21 males and 24 females, ranging in age from 22-83 yr old. No sex differences were shown in the stimulation of enzyme release for either age group. However, stimulation was found to significantly decline in both males and females over 50 yr old compared to subjects under 50 yr old. The linear formulae for .beta.-glucuronidase, .beta.-galactosidase and lysozyme in male subjects were Y = 6.5X + 617.2, Y = -1.9X + 311.5 and Y = -1.9X + 327.3 with correlation coefficient of -0.685, -0.352 and -0.401, respectively. The linear formulae in females were Y = -5.2X + 536.6, Y = -3.0X + 340.6 and Y = -1.7X + 333.6 with correlation coefficient of -0.582, -0.303 and -0.462, respectively. There apparently was an age-related decline of response to the stimulant, fMet-Leu-Phe.