Fungal metabolism and detoxification of the nitropolycyclic aromatic hydrocarbon 1-nitropyrene
- 1 September 1985
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 50 (3) , 649-655
- https://doi.org/10.1128/aem.50.3.649-655.1985
Abstract
Nitropolycyclic aromatic hydrocarbons are ubiquitous environmental pollutants, many of which are potent mutagens in bacterial and mammalian cells and carcinogenic to rodents. In this study, we investigated the fungal metabolism of 1-nitropyrene and determined the mutagenic activity of the metabolites toward Salmonella typhimurium TA98, TA98NR, and TA100. Cunninghamella elegans metabolized 1-nitropyrene to form glucoside conjugates of 6-hydroxy-1-nitropyrene and 8-hydroxy-1-nitropyrene. The metabolites were isolated by reversed-phase high-pressure liquid chromatography and characterized by application of UV absorption, 1H-nuclear magnetic resonance, and mass spectroscopy. Mutagenicity assays performed on samples extracted from incubations of C. elegans with 1-nitropyrene indicated that mutagenic activity decreased with time. Consistent with the loss in mutagenic activity, the glucoside conjugates of 6- and 8-hydroxy-1-nitropyrene were nonmutagenic in the Salmonella reversion assay. The results indicate that the fungus C. elegans metabolizes 1-nitropyrene to detoxified products.This publication has 45 references indexed in Scilit:
- 1-nitrosopyrene: An intermediate in the metabolic activation of 1-nitropyrene to a mutagen in Salmonella typhimurium TA1538☆Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1985
- Metabolism of nitropolycyclic aromatic hydrocarbons by human intestinal microfloraBiochemical and Biophysical Research Communications, 1984
- Metabolism of 1,8-dinitropyrene by Salmonella typhimuriumChemico-Biological Interactions, 1984
- Nitration of carcinogenic and non-carcinogenic polycyclic aromatic hydrocarbons results in products able to induce transformation of Syrian hamster cellsCarcinogenesis: Integrative Cancer Research, 1983
- Transformation of normal human skin fibroblasts by 1-nitropyrene and 6-nitrobenzo [a] pyreneCarcinogenesis: Integrative Cancer Research, 1983
- The 1-nitropyrene reductase of Salmonella typhimuriumChemico-Biological Interactions, 1983
- Metabolism of the mutagenic environmental pollutant, 6-nitrobenzo[a]pyrene: Metabolic activation via ring oxidationBiochemical and Biophysical Research Communications, 1982
- Xanthine oxidase catalyzed binding of 1-nitropyrene to DNABiochemical and Biophysical Research Communications, 1982
- Metabolism of 1-nitropyrene and formation of DNA adducts in Salmonella typhimuriumCarcinogenesis: Integrative Cancer Research, 1981
- Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1975