Studies of an Inhibitor of the Two Antibody Immunoassay System
- 1 January 1964
- journal article
- research article
- Published by American Diabetes Association in Diabetes
- Vol. 13 (1) , 1-5
- https://doi.org/10.2337/diab.13.1.1
Abstract
A heat labile plasma inhibitor of the two antibody insulin immunoassay system has been investigated by means of paper electrophoresis, ultracentrifugation, and DEAE cellulose anion exchange resin. These studies indicate that the inhibitor does not interfere with the binding of insulin to its specific antibody (first antibody reaction); but that the inhibitor interferes with the reaction of anti-guinea pig serum (rabbit source) with the insulin-anti-insulin soluble complex (second antibody reaction). When plasma samples are appropriately diluted the effect of the inhibitor is minimized.Keywords
This publication has 5 references indexed in Scilit:
- Immunoassay of Insulin: Two Antibody System: Plasma Insulin Levels of Normal, Subdiabetic and Diabetic RatsDiabetes, 1963
- Immunoassay of Insulin Using a Two-Antibody System.Experimental Biology and Medicine, 1962
- STUDIES ON HUMAN GROWTH HORMONE. I. A RADIO-IMMUNOASSAY FOR HUMAN GROWTH HORMONE*Journal of Clinical Investigation, 1962
- Nonprecipitating Insulin Antibodies12Journal of Clinical Investigation, 1958
- INSULIN-I131 METABOLISM IN HUMAN SUBJECTS: DEMONSTRATION OF INSULIN BINDING GLOBULIN IN THE CIRCULATION OF INSULIN TREATED SUBJECTS 1Journal of Clinical Investigation, 1956