Transport of horseradish peroxidase from the cell surface to the Golgi in insulin-secreting cells: preferential labelling of cisternae located in an intermediate position in the stack.

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Abstract
We have used serial sectioning to study the topology of Golgi cisternae in insulin‐secreting cells during secretion‐stimulated endocytotic uptake of exogenous horseradish peroxidase (HRP). HRP‐labelled cisternae were followed on several series of consecutive sections. This revealed that labelled cisternae could always be traced to a position in the Golgi stack intermediate between the cis and the trans poles. This occurred in spite of the apparent cis or trans locations of HRP‐containing cisternae on some sections. The latter images could be explained by the lack of the true cis or trans (clathrin‐coated) cisternae at certain levels of the stack.