One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins
Open Access
- 1 September 1996
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 24 (17) , 3469-3471
- https://doi.org/10.1093/nar/24.17.3469
Abstract
With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.Keywords
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