Phorbol 12‐myristate 13‐acetate modulates the cAMP‐induced light‐scattering response of a Dictyostelium discoïdeum cell population
Open Access
- 5 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 241 (1-2) , 149-153
- https://doi.org/10.1016/0014-5793(88)81049-4
Abstract
The effect of phorbol 12‐myristate 13‐acetate upon the light‐scattering response to cAMP of a D. discoïdeum cell suspension was investigated. It was found that the first spike of the cAMP‐mediated light‐scattering change (peaking at about 15–20 s after stimulation) was inhibited by the phorbol ester. This effect was concentration dependent with an half‐maximum value for the inhibition of 4 nM. The inhibition was found to be maximal after a 10–20 min incubation time. The phorbol ester was shown to affect the dose‐response relationship between the cAMP concentration and the relative amplitude of the light‐scattering change, more by decreasing the number of cAMP receptors than by decreasing their apparent affinity for cAMP.Keywords
This publication has 20 references indexed in Scilit:
- Altered cyclic-AMP receptor activity and morphogenesis in a chemosensory mutant of Dictyostelium discoideumDifferentiation, 1986
- Inositol 1,4,5-trisphosphate induces calcium release from a non-mitochondrial pool in amoebae of DictyosteliumBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Studies and Perspectives of Protein Kinase CScience, 1986
- Selective down-regulation of cell surface cAMP-binding sites and cAMP-induced responses in Dictyostelium discoideumBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985
- cAMP activates adenylate and guanylate cyclase of Dictyostelium discoideum cells by binding to different classes of cell-surface receptors. A study with extracellular Ca2+Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985
- Guanine nucleotides modulate cell surface cAMP-binding sites in membranes from Dictyostelium discoideumBiochemical and Biophysical Research Communications, 1984
- A study on sensing and adaptation in Dictyostelium discoideum: guanosine 3',5'-phosphate accumulation and light-scattering responses.The Journal of cell biology, 1983
- Chemotaxis in DictyosteliumAnnual Review of Physiology, 1982
- Signal transduction in the cellular slime moldsMolecular and Cellular Endocrinology, 1982
- Streamers: Chemotactic Mutants of Dictyostelium discoideum with Altered Cyclic GMP MetabolismMicrobiology, 1981