Two anonymous X-specific human sequences detecting restriction fragment length polymorphisms in region Xq26?qter
- 1 November 1984
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 10 (6) , 607-613
- https://doi.org/10.1007/bf01535226
Abstract
Two anonymous X-specific sequences isolated from a genomic library of flow-sorted X chromosomal DNA were selected for study because they revealed restriction fragment length polymorphisms in the region Xq26 → qter. One sequence, DXS10, detected a two-allele TaqI polymorphic system with allele frequencies of 0.33 and 0.67. The other, 4D-8, defined an Mspl polymorphism with allele frequencies of 0.18 and 0.82. DXS10 is tightly linked to the hypoxanthine phosphoribosyltransferase (HPRT)locus with recombination distance θ=0 cM at LOD=5.55 (95 % probability limit θ HPRTlocus itself. 4D-8 shows no detectable linkage to the HPRTlocus, with maximum likelihood estimate for θ=50 cM and a LOD score of −2.61 at θ= 5 cM. These two polymorphisms provide additional chromosomal loci for gene mapping by linkage at the distal end of the long arm of the human X chromosome.Keywords
This publication has 26 references indexed in Scilit:
- Report of the committee on human gene mapping by recombinant DNA techniquesCytogenetic and Genome Research, 1984
- Close linkage of fragile X-mental retardation syndrome to haemophilia B and transmission through a normal maleNature, 1983
- Human hypoxanthine-guanine phosphoribosyltransferase. Detection of a mutant allele by restriction endonuclease analysis.Journal of Clinical Investigation, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Detection of heterozygous carriers of the Lesch-Nyhan syndrome by electrophoresis of hair root lysatesThe Journal of Pediatrics, 1973
- LYON HYPOTHESIS AND X-LINKED DISEASEThe Lancet, 1967
- A familial disorder of uric acid metabolism and central nervous system functionThe American Journal of Medicine, 1964
- Genetical linkage between the loci for glucose‐6‐phosphate dehydrogenase deficiency and colour‐blindness in American NegroesAnnals of Human Genetics, 1962
- Gene Action in the X-chromosome of the Mouse (Mus musculus L.)Nature, 1961