Membrane lateral pressure as a modulator of glycerol‐3‐phosphate dehydrogenase activity
- 1 October 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 271 (1-2) , 165-168
- https://doi.org/10.1016/0014-5793(90)80398-3
Abstract
Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the enzyme activity, namely V max value. Contrarily, changes in surface charge caused by minute amounts of phosphatidylserine or charged organic substances influenced only the apparent K m value. The role of bulk phospholipids in regulation of glycerol-3-phosphate dehydrogenase is discussed.Keywords
This publication has 26 references indexed in Scilit:
- Mg2+-induced changes of lipid order and conformation of (Na+ + K+)-ATPaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- Factors affecting the reaggregation of rat brain microsomes solubilized with octyl glucoside and their relationship with the base-exchange activity of reaggregatesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- Regulation of glycerol 3‐phosphate oxidation in mitochondria by changes in membrane microviscosityFEBS Letters, 1986
- Effect of Phospholipid Composition on the Surface Potential of Liposomes and the Activity of Enzymes Incorporated into LiposomesEuropean Journal of Biochemistry, 1980
- Fluidity parameters of lipid regions determined by fluorescence polarizationBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1978
- Regulation of membrane enzymes by lipidsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1978
- The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatographyBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- Regulation of allosteric membrane-bound enzymes through changes in membrane lipid compositionBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1975
- The regulation of glycerol 3-phosphate oxidase of rat brownadipose tissue mitochondria by long-chain free fatty acidsMolecular and Cellular Biochemistry, 1975
- UNUSUALLY HIGH MITOCHONDRIAL ALPHA GLYCEROPHOSPHATE DEHYDROGENASE ACTIVITY IN RAT BROWN ADIPOSE TISSUEThe Journal of cell biology, 1969