Studies on microbial RNA, 3. Formation of submethylated sRNA in Saccharomyces cerevisiae.

Abstract
Growth conditions, which result in the formation of submethylated sRNA are described for a methionlne-requiring yeast mutant. This sRNA was assayed with S-adenosylmethionine and a partly-purified methylase from yeast. The reaction product was characterized, and thymine was the main methylated base formed. Actidione treatment of methionine-starved yeast caused the submethylated sRNA to disappear.

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