High Production of D-Tagatose, a Potential Sugar Substitute, Using Immobilized L-Arabinose Isomerase

2 February 2001

journal article
Published by Wiley in Biotechnology Progress

Vol. 17 (1) , 208-210
https://doi.org/10.1021/bp000147u

Abstract

An L‐arabinose isomerase of Escherichia coli was immobilized using covalent binding to agarose to produce D‐tagatose, a bulking sweetener that can be economically used as a sugar substitute. The immobilized L‐arabinose isomerase stably produced an average of 7.5 g‐tagatose/L·day for 7 days with a productivity exceeding that of the free enzyme (0.47 vs 0.30 mg/U·day). Using a scaled‐up immobilized enzyme system, 99.9 g‐tagatose/L was produced from galactose with 20% equilibrium in 48 h. The process was repeated two more times with production of 104.1 and 103.5 g‐tagatose/L. D‐Tagatose production using an immobilized L‐arabinose isomerase has a high potential for commercial application.

Keywords

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