Eukaryotic transcriptomics in silico: Optimizing cDNA-AFLP efficiency
Open Access
- 1 January 2009
- journal article
- research article
- Published by Springer Nature in BMC Genomics
- Vol. 10 (1) , 565
- https://doi.org/10.1186/1471-2164-10-565
Abstract
Complementary-DNA based amplified fragment length polymorphism (cDNA-AFLP) is a commonly used tool for assessing the genetic regulation of traits through the correlation of trait expression with cDNA expression profiles. In spite of the frequent application of this method, studies on the optimization of the cDNA-AFLP assay design are rare and have typically been taxonomically restricted. Here, we model cDNA-AFLPs on all 92 eukaryotic species for which cDNA pools are currently available, using all combinations of eight restriction enzymes standard in cDNA-AFLP screens.This publication has 37 references indexed in Scilit:
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