Comparative analysis of CD8 expressed on mature CD4+ CD8+ T cell clones cultured with IL-4 and that on CD8+ T cell clones: implication for functional significance of CD8β

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Abstract
Interleukln (IL-4) can induce CD8 expression on mature CD4+ T cells. To study this phenomenon in more detail, we characterized CDS expressed on IL-4-lnduced CD4 + CD8+ (double positive) T cell clones in comparison with that on CD8+ T cell clones. Using 2ST8–5H7 mAb that detects CD8β expression, we found that double positive T cell clones isolated with IL-4 express CD8α but not β, in contrast to CD8+ CTL cell clones, which express both chains of CD8. Northern blot analysis revealed that these double positive clones expressed CD8α but not β mRNA, indicating that CD8α and β are Independently regulated at the pre-translatlonal level. Immunopreclpitation experiments showed that CD8 expressed on a representative IL-4-lnduced double positive T cell clone consists mainly of homodimers of a single 34 kd protein of CD8α. The amount of multimers detected from this clone was much less than that from a CD8+ CTL clone. These results suggest that persistent expression of CD8β Is specific for the CD8+ lineage and may be involved in polymerization and stabilization of CD8 which enhances the efficiency of class l-restrlcted antigen recognition.

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