Comparative reduction/oxidation studies with single chain des‐(B30) insulin and porcine proinsulin
- 1 April 1985
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 25 (4) , 431-434
- https://doi.org/10.1111/j.1399-3011.1985.tb02197.x
Abstract
The single chain des(B30) insulin molecule (SCI) was reduced and reoxidized together with porcine proinsulin (PPI). Yields of correctly folded and reoxidized SCI and PPI were analyzed by HPLC [high performance liquid chromatography]. The concentrations of both proteins were 10-3 M during reduction and 10-5 M during oxidation. The pH during reoxidation was varied from 8.6 to 9.2 and the temperature from 4 to 37.degree.. Under all conditions tested, the recovery of SCI was substantially higher than that of PPI. The recoveries peaked after 24-72 h. Apparently the miniproinsulin SCI folds correctly up more efficiently than porcine proinsulin, resulting in higher yields of reoxidized SCI.Keywords
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