Automated cation-exchange chromatography of peptides at a high sensitivity (0–0.1 absorbance range) (Short Communication)

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Abstract
A method is described where leaching of the column resin by pyridine–acetate buffers is minimal and excessive rise of the base-line of the chromatogram is avoided. The method can be used to fractionate peptide mixtures at a concentration of 2–20nmol/component.

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