Evidence for 16β-hydroxylation of estrogens by guinea pig liver slices

Abstract
In an earlier communication, it was shown that estrone-3-sulfate could be hydroxylated in the 16.alpha. position by guinea pig liver slices. Disulfates of 16.alpha.-hydroxyestrone and estriol were identified. Suggestive evidence for 6-hydroxylated metabolites was mentioned. A careful reinvestigation showed that the steroid disulfate fraction is composed of 16.alpha.- and 16.beta.-hydroxylated steroids. 16.beta.-Hydroxyestrone in particular is an important quantitative metabolite of estradiol-17.beta.. No firm evidence is available for the formation of 6-hydroxysteroids in this tissue system.

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