φC31 Integrase Confers Genomic Integration and Long-Term Transgene Expression in Rat Retina
- 1 June 2005
- journal article
- Published by Association for Research in Vision and Ophthalmology (ARVO) in Investigative Opthalmology & Visual Science
- Vol. 46 (6) , 2140-2146
- https://doi.org/10.1167/iovs.04-1252
Abstract
Purpose. Gene therapy has shown promise in animal models of retinal disease, with the most success achieved to date with viral vectors used for gene delivery. Viral vectors, however, have side effects and limitations and are difficult to manufacture. The present study was conducted in an attempt to develop a novel system for long-term gene transfer in rat retinal pigment epithelium (RPE), by using nonviral transfection methods for gene transfer and the integrase from the bacteriophage φC31 to confer long-term gene expression by means of genomic integration.Keywords
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