Effects of tumour necrosis factor ? on bone marrow aspirates of patients with acute myelogenous leukemia determined by flow-cytometric cell-cycle analysis
- 1 January 1992
- journal article
- research article
- Published by Springer Nature in Zeitschrift für Krebsforschung und Klinische Onkologie
- Vol. 118 (1) , 56-60
- https://doi.org/10.1007/bf01192312
Abstract
Tumour necrosis factor α (TNFα) exerts cytotoxic and antiproliferative effects on neoplastic cells. It has been used as a therapeutic agent for solid tumours and haematological malignancies. We report on the ex vivo determination of the effect of recombinant human rhuTNFα on bone marrow aspirates by a bromodeoxyuridine/propidium iodide method. Cell samples were drawn after 0.5, 2, 4, 6, 8, 10, 22, and 25 h from shortterm suspension bone marrow cultures from patients with acute myelogenous leukemia (AML). Flow-cytometric cell-cycle analysis was performed after double DNA staining with propidium iodide and anti-BrdU antibodies. By this method the effect of rhuTNFα on cell proliferation can be evaluated after only 35 h. In about two-thirds of the bone marrow aspirates of AML an inhibiting effect on rhuTNFα can be demonstrated, developing to its full extent after 10 h.Keywords
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